l1210 murine lymphoblastic leukemia cells  (ATCC)

Journal: Toxicology in vitro : an international journal published in association with BIBRA

Article Title: Involvement of extrinsic and intrinsic apoptotic pathways together with endoplasmic reticulum stress in cell death induced by naphthylchalcones in a leukemic cell line: advantages of multi-target action.

doi: 10.1016/j.tiv.2014.02.002

Figure Lengend Snippet: Fig. 2. Apoptosis induction in L1210 cells by chalcones. The apoptosis was measured by Annexin-V-FITC/PI) staining. L1210 cells were treated with chalcones for 12 h, stained with the dyes and analyzed by flow cytometry. The horizontal and vertical axes represent labeling with Annexin-V-FITC and PI, respectively. Q1 represents live cells, Q2 represents early apoptotic cells (positive for Annexin V only), Q3 represents late apoptotic cells (Annexin-V and PI positives) and Q4 represents necrotic cells (positive for PI only). The dot plots shown (a) are representative of one experiment, and the graph (b) shows the mean ± SEM of three independent experiments. ⁄⁄ p < 0.01 compared with the control.

Article Snippet: Murine L1210 lymphoblastic leukemia cells, murine fibroblast (NIH/3T3) and monkey kidney cells (VERO) were obtained from the American Type Culture Cell (ATCC).

Techniques: Staining, Cytometry, Labeling, Control

Journal: Toxicology in vitro : an international journal published in association with BIBRA

Article Title: Involvement of extrinsic and intrinsic apoptotic pathways together with endoplasmic reticulum stress in cell death induced by naphthylchalcones in a leukemic cell line: advantages of multi-target action.

doi: 10.1016/j.tiv.2014.02.002

Figure Lengend Snippet: Fig. 3. Activation of caspase-3,-8 and -9 in L1210 cells by chalcones. L1210 cells were incubated with the compounds for 4 h. Caspases activities were measured by monitoring the cleavage of Ac-DEVD-AMC, a fluorogenic substrate for caspase-3, Ac-IETD-AMC, a fluorogenic substrate for caspase-8 and of Ac-LEHD-AFC a fluorogenic substrate for caspase-9. The activity is given as a percentage of the control considered 100%. ⁄ p < 0.05 and ⁄⁄ p < 0.01 compared with the control.

Article Snippet: Murine L1210 lymphoblastic leukemia cells, murine fibroblast (NIH/3T3) and monkey kidney cells (VERO) were obtained from the American Type Culture Cell (ATCC).

Techniques: Activation Assay, Incubation, Activity Assay, Control

Journal: Toxicology in vitro : an international journal published in association with BIBRA

Article Title: Involvement of extrinsic and intrinsic apoptotic pathways together with endoplasmic reticulum stress in cell death induced by naphthylchalcones in a leukemic cell line: advantages of multi-target action.

doi: 10.1016/j.tiv.2014.02.002

Figure Lengend Snippet: Fig. 8. Effect of chalcones on cell cycle distribution. L1210 cells were treated with chalcones and Paclitaxel for 12 (a) and 24 h (b) and stained with PI. Following flow cytometry, cellular DNA profile was further analyzed using the software WinMDI 2.9. Data represent the percentage of cell counts in each cell cycle phase. ⁄ p < 0.05 and ⁄⁄

Article Snippet: Murine L1210 lymphoblastic leukemia cells, murine fibroblast (NIH/3T3) and monkey kidney cells (VERO) were obtained from the American Type Culture Cell (ATCC).

Techniques: Staining, Cytometry, Software